Separation of host cells and impurities from the output of fermentation in alkaloid drugs bioreactor by ultrafiltration

Research

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Proposal Title:

Separation

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of

host

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cells

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and

impurities

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from the output of fermentation in

alkaloid

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drugs

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bioreactor

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by

ultrafiltration

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Background:

Alkaloid

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drugs

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are a wide organic compound which are made by living organisms. Alkaloids have at least nitrogen atoms in its structure and have a wide range of phrenological effects

such

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as antibacterial and antimalaria. Nowadays, a myriad of

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focuses on the industrial fermentative

production

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of

alkaloid

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drugs

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from natural sources.

However

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, it is relatively easy to ferment the sources, the low concentration of desired compounds makes drug discovery from fermentation

bioreactor

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difficult.

Therefore

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, others

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concentrates on downstream processing which is costly part in bio-

drugs

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production

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. Downstream processing entails the recovery bio-product from natural sources

such

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as

host

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cells

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and elimination

impurities

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from complex fermentation

broth

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in order to recycle of salvageable materials. The output of fermentation

bioreactor

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is a mixture of diverse molecules which have different in the physical and chemical properties.

Therefore

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, downstream processing typically determined by economic feasibilities of the approach.

Ultrafiltration

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and microfiltration are a common step in

separation

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of

host

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cell and

impurities

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because of high selectivity and low operation conditions. The

ultrafiltration

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membrane

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can be combined by fermentation

bioreactor

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so as to increase the productivity of the fermentation.

Ultrafiltration

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is a

membrane

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separation

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process

that is

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used in a vast wastewater and food industries

such

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as dairy.

Ultrafiltration

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is the most convenient

membrane

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for setting the level of protein in milk and whey.

Also

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, the all protein and solids in milk which have molecular weight higher than fat globules are eliminated by

ultrafiltration

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membrane

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.

Ultrafiltration

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are be installed before solvent extraction in order to increase solvent extraction efficiency by avoiding emulsification Li et al., 2004.

Moreover

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, the two-step

ultrafiltration

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is used in purification of benzylpenicillin from fermented

broth

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by Nabais and Cardoso (1999).

This

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research

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evaluated three

ultrafiltration

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with molecular weight cut-off (

MWCO

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) 8000,20000 and 10000. The result show high benzylpenicillin recovery and elimination proteins and

impurities

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. Researchers have reported

ultrafiltration

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of hemicellulose hydrolysate fermentation

broth

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for xylitol

production

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which is food additive and sugar substitutes. The

ultrafiltration

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membrane

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with 10000

MWCO

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shows the best flux and xylitol concentration in permeate. M. T. A. P. Kresnowati, Ria Desiriani and I. G. Wenten

This

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research

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is part of develop a method of

alkaloid

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drugs

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production

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in Australia company, that focuses on utilization of

ultrafiltration

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for

separation

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of

host

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cells

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and

impurities

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from fermentation

broth

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in downstream

production

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. Methodology: The objects of these

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are to study the performance of

ultrafiltration

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on

host

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cell and

impurities

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separation

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and to evaluate the effects of

membrane

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MWCO

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, transmembrane pressure, cross-flow velocity on permeate flux. 1.The experiments will conduct on the following step: 2.Preparing a fermentation

broth

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of

alkaloid

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bioreactor

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from Australian company. 3.Design and install the

ultrafiltration

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membrane

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system includes recirculation systems,

ultrafiltration

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modules and online permeate weight measuring. 4.Preparing and characterizing the

ultrafiltration

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membrane

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with a different ingredient and

MWCO

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. 5.Conducting the experiment and collecting result from diverse condition. 6.Analyzing the experiment results and calculating the permeate flux and the retention ratio. Expected outcome:

This

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research

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will carry out to establish the highest performance of

ultrafiltration

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membrane

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for

separation

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host

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cells

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and

impurities

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from output of

alkaloid

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fermentation

bioreactor

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in optimal operation conditions.

Moreover

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, the desired

ultrafiltration

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membrane

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will be evaluated in other operation condition and fouling of

membrane

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. It is expected that productivity of

alkaloid

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drugs

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increases with the results of

this

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research

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.