Knowledge of sex identification and reproduction outputs in fish still requires more details to optimize aquaculture facilities management, particularly on some species, they are mature later than the male, or hermaphrodite species with a sex ratio managing reason, etc. (Barkowski and Haukenes, 2014; Budd et al., 2015; Chen et al., 20201-3). A relevant low-risk method is become an important option to determine reproduction outputs based on sex and maturity status. Although several methods have been used, so far, in sex and maturity identification, including ultrasonography (Moghim et al., 2002; Kohn et al., 20134-5), laparoscopy (Falahatkar et al., 20116), plasma sex steroid assay (Mandich et al., 2004; Kohn et al., 2013; de Carvalho et al., 20175, 7-8) and gonad histology (Petochi et al., 2011; Falahatkar and Poursaeid, 20149-10), some of them are considered as invasive techniques. Among the different procedures, blood sampling is usually regarded as a robust source of reproduction...