The story of activation is best seen in Figures 1 and 2. Figure 1 clearly tells us that mixing purified αq with AlF4- and a brain enzyme caused a sharp rise in InsP3. This shows that the purified αq is enough to activate the target enzyme PLC. This activated αq then worked together with phospholipase C, causing a clear rise in IP3. This clearly proved that activating phospholipase C was αq’s unique role. Authors says that “when we used in the same assay, neither purified βγ subunits or the … affected the activity of PI-PLC”. Figure 2 looks at calcium’s role, showing that αq worked at every concentration tested. Most notably, it changed the enzyme’s calcium sensitivity, shifting its affinity from ~ 1 μM to 0.1 μM. This suggests that αq doesn’t just amplify the signal, instead it refines the sensitivity of the system, making the intrinsic activity of PLC more potent.