Zoonoses are communicable diseases transmitted from animals to humans. More than 60% of human infectious diseases are caused by pathogens shared with wild or domestic animals. Emerging zoonoses are a growing threat to global health. Lyme borreliosis (LB) is the most prevalent tick-borne zoonosis in the northern hemisphere including Slovakia. It is caused by spirochetes belonging to the Borrelia burgdorferi (B.b.)sensu lato (s.l.) complex which are transmitted by ticks . B.afzelii and B. garinii are the most common European circulatinggenospecies The routine diagnosis of borreliosis in humans is based on the determination of the levels of specific antibodies using the enzyme-linked immunosorbent assay (ELISA). If the first step is reactive, immunoblotting is performed to confirm it. IgG antibodies appear in serum 6 weeks after the infection, they reach their peak level after 4 to 6 months and are detectable in serum for many years. Tularaemia (TUL), also known as “rabbit fever”, is...